Plant Transcription Factor Database
Previous version: v3.0
Oryza sativa subsp. indica
HSF Family
Species TF ID Description
BGIOSGA003800-PAHSF family protein
BGIOSGA004025-PAHSF family protein
BGIOSGA004402-PAHSF family protein
BGIOSGA004459-PAHSF family protein
BGIOSGA006348-PAHSF family protein
BGIOSGA006438-PAHSF family protein
BGIOSGA007838-PAHSF family protein
BGIOSGA009826-PAHSF family protein
BGIOSGA010671-PAHSF family protein
BGIOSGA011509-PAHSF family protein
BGIOSGA011916-PAHSF family protein
BGIOSGA012151-PAHSF family protein
BGIOSGA013724-PAHSF family protein
BGIOSGA016941-PAHSF family protein
BGIOSGA020240-PAHSF family protein
BGIOSGA021054-PAHSF family protein
BGIOSGA023100-PAHSF family protein
BGIOSGA025247-PAHSF family protein
BGIOSGA026239-PAHSF family protein
BGIOSGA026537-PAHSF family protein
BGIOSGA026829-PAHSF family protein
BGIOSGA029403-PAHSF family protein
BGIOSGA029640-PAHSF family protein
BGIOSGA030901-PAHSF family protein
BGIOSGA031910-PAHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645